Naren November 16

Sorting to Mitochondria and Peroxisomes
Lecture 3 has been reposted. He is attempting to post the movies.
Not testing on this, but it is interesting.
3:
talking about transport of cytosolic protein into peroxisome or mitochondria.
Mitochondrial Import
mitochondrial inner membrane has large surface area. Inner membrane is nearly 75% protein. Everything goes through active transport. Matrix is not hollow- has lots of proteins.
There is a unique feature to the mitochondrial targeting sequence- an amphipathic helix.
11:
What it looks like.
Experiment to find the sequence- slide 13.
Mitochondrion has 2 sets of pores- Tom complex, TIM23, TIM22, OXA.
23: 1. keeps protein unfolded.
18:
If protein is kept unfolded, introduce a linker, molecule penetrates membrane. Add methatrexate, binds active site DHFR- folds and gets stuck. 50 AAs was best linker. Labelled using immunogold.
Targeting sequence:
table slide 32.
Peroxisomes defined:
35.
Peroxisomes are strictly eukaryotic.
Peroxisome has one bilayer. Present largely in liver cells for detoxification.
37: PTS2 is not well defined.